Effect of Seminal Zinc, Calcium, Oxidative Stress and Protein Profile on Semen Quality of Crossbred Bulls

The objective of present study was to investigate the effect of endogenous minerals (Zn and Ca), seminal proteins and oxidative stress on semen quality of crossbred bulls. Two crossbred bulls with history of good initial quality, high sperm motility percentage, and freezable ejaculates and poor initial quality, low sperm motility percentage, and donating mostly non-freezable ejaculates (Bull B), respectively were utilized. Six ejaculates from each bull were used and categorized into high progressive motile as good quality and low progressive motile as poor quality ejaculates groups. Total 24 ejaculates were taken during entire period of study. The level of Zn, Ca in seminal plasma and Ca in sperm pellets was found significantly (P<0.05) higher in good quality ejaculates of Bull A compared to poor quality ejaculates of Bull B; however, the level of reactive oxygen species and malondialdehyde was significantly higher (P<0.05) in poor quality ejaculates of Bull B compare to good quality ejaculate of Bull A. The 25 kDa protein band was prominent only in good quality ejaculate of Bull A. It was concluded that several proteinaceous antioxidant enzymes which may be present in 25 kDa band and minerals like Zn and Ca as a cofactors of these enzymes could be responsible for good quality semen ejaculates of Bull A

Indications and effectiveness of arthroscopic lavage and debridement in osteoarthritis knee

Background: Degenerative joint disease will afflict most of the people if they live long enough. The majority of patients with osteoarthritis present to orthopaedic surgeons seeking relief of pain and associated restoration of function. Various intraarticular and periarticular structures may be the cause of pain. Improvement in osteoarthritis by arthroscopic lavage and debridement is seen due to various mechanisms.Methods: In a prospective study from July 2016 to December 2018, 35 patients with primary osteoarthritis knee were assessed for effectiveness of arthroscopic lavage and debridement in relieving symptoms of osteoarthritis of knee and to determine the indications of arthroscopy in osteoarthritis of knee. Assessment was done using variables as age of the patient, body mass index, varus deformity, radiographic grading and arthroscopic grading.Results: A declining trend was seen on follow up over time; 91.4% excellent to good results seen at one month follow up, 76.1% at six months, 49.93% at twelve months, 37.5%, at eighteen months, 23.07% at twenty-four months and 28.5% at thirty months. Results at six month follow up when compared, were better for age less than 50 years (88.8% Vs 73.1% in >50 years age), normal weight patients with BMI 18.5 to 25(94.5% Vs 58.5% in overweight), varus angulation <100(93.1% Vs 0% in >100), radiological grade I and II (95-100% Vs 45-50% in grade III and IV) and arthroscopic grade I and II (94-100% Vs 0-77% in grade III and IV).Conclusions: Arthroscopic lavage and debridement is an effective method of treatment for osteoarthritis knee in patients with grade I and grade II osteoarthritis having symptoms of pain and locking due to loose bodies or degenerative meniscal tears.

Fingertip reconstruction: study of functional and cosmetic results of various local flaps and grafting techniques in patients attending a tertiary care center in North India

Background: Finger injuries are becoming more common with the increasing use of mechanical, industrial, and household appliances. There are six main types of repair for this injury. The replacement of skin loss in digital injuries, particularly on the volar aspects, is an important part of hand-injury management. It would be of value to know the best type of cover, especially for sensory reinnervation as well as for subjective evaluation, cosmetic appearance and freedom from complications. The objective of this study was to measure incidence of age, sex, site, pattern of injury as well as to evaluate and compare the functional and cosmetic results of the various local flap and grafting techniques in finger reconstruction distal to metacarpophalangeal joint.Methods: In a prospective study between May 2014 to October 2018 consisting of 50 patients (43 males, 7 females), aged between 2 years and 54 years, with soft tissue loss distal to metacarpophalangeal joint were treated with either free split-thickness skin grafts, palmar flaps, cross-finger flap or pedicle flaps from the abdomen. The follow-up period ranged from 10 days to 15 months from the date of surgery. Subjective and objective evaluation was done and graded as excellent, good, fair and poor.Results: In this series there were 2 good and 6 fair results for split skin grafting; 9 good and 6 fair results for v-y plasty; 5 excellent, 12 good and 4 fair results for cross finger flap; 1 good and 1 fair result for radial artery based flap; 1 excellent and 1 good for first metacarpal artery based flap and 2 good results for abdominal flap. Overall 6(12%) had excellent, 27(54%) had good and 17(34%) had fair results. None of them had poor results.Conclusions: The group of patients with cross-finger flaps had less subjective complaints and more normal objective testing results than the other groups. When an extensive tactile pad avulsion exists, the cross-finger pedicle flap offers the best long-term result with fewer secondary problems. The exclusive use of any one method cannot be advocated since each serves an useful purpose under proper conditions.

Neonatal morbidity and care-seeking behavior in urban Lucknow.

We conducted this study to assess the neonatal morbidity and care-seeking behavior amongst slum and non-slum dwellers at Lucknow. One hundred and fifty neonates were recruited within 48 hours of birth from an urban Reproductive and Child Health center and followed up at 6 weeks +/- 15 days at home. Twenty five (16.6%) were lost to follow-up. Among those followed up, 46.4% (58/125) developed one or more morbidity; 26% (15/58) of these did not receive qualified medical care. Neonatal morbidity was 56.8% (33/58) among slum dwellers and 37.3% (25/67) among non-slum dwellers (absolute difference=19.5%, 95% CI=3.3 to 34.7; P=0.04). Severe neonatal illnesses were also significantly higher among neonates from slums as compared to those from non-slum areas (OR=4.50, 95% CI=1.28 to 16.38, P=0.007). Male gender was associated with any care-seeking (OR=1.24, 95% CI =1.24 to 91.99; P=0.03) and was more likely to be seen by a qualified provider (OR=3.8, 95% CI=1.05 to 13.94; P=0.04). Since nearly half of the neonates had morbidity and more than a quarter of them did not receive qualified medical care, there is a need to introduce Community Integrated Management of Neonatal and Childhood Illnesses (IMNCI) program here, emphasizing on the importance of qualified medical care for ill neonates, including females.

Familial Behcet's disease.

There are very few reports of Behetaet's disease from India. Familial aggregation of Behetaet's disease has been reported with restricted geographical distribution. We report here familial Behcet's disease from India in two brothers aged 30 and 32 years. Both patients had recurrent oral and genital ulcers for approximately five years. They also had arthralgias on and off along with fever. Pathergy test was positive in both cases. Their younger brother and a sister had recurrent oral aphthous ulcers.